02448nas a2200385   4500000000100000000000100001008004100002260001200043653006600055653001700121653002300138653001100161653001600172653001900188653003100207653000900238653002300247100002000270700001800290700001900308700001600327700001800343700002700361700001500388700002000403700002600423700001600449700002100465700002100486245007400507300001400581490000700595520144600602022001402048       2023                            d  c2023-1110aAllergic contact dermatitis; human predictive patch test data10aBenchmarking10aDatabases, Factual10aHumans10aPatch Tests10aReference data10aReproducibility of Results10aSkin10aSkin sensitization1 aJudy Strickland1 aJaleh Abedini1 aDavid G. Allen1 aJohn Gordon1 aVictoria Hull1 aNicole C. Kleinstreuer1 aHon-Sum Ko1 aJoanna Matheson1 aHermann-Josef Thierse1 aJames Truax1 aJens T. Vanselow1 aMatthias Herzler00aA database of human predictive patch test data for skin sensitization  a2825-28370 v973 aCritical to the evaluation of non-animal tests are reference data with which to assess their relevance. Animal data are typically used because they are generally standardized and available. However, when regulatory agencies aim to protect human health, human reference data provide the benefit of not having to account for possible interspecies variability. To support the evaluation of non-animal approaches for skin sensitization assessment, we collected data from 2277 human predictive patch tests (HPPTs), i.e., human repeat insult patch tests and human maximization tests, for skin sensitization from 1555 publications. We recorded protocol elements and positive or negative outcomes, developed a scoring system to evaluate each test for reliability, and calculated traditional and non-traditional dose metrics. We also traced each test result back to its original report to remove duplicates. The resulting database, which contains information for 1366 unique substances, was characterized for physicochemical properties, chemical structure categories, and protein binding mechanisms. This database is publicly available on the National Toxicology Program Interagency Center for the Evaluation of Alternative Toxicological Methods website and in the Integrated Chemical Environment to serve as a resource for additional evaluation of alternative methods and development of new approach methodologies for skin sensitization assessments.  a1432-0738