02300nas a2200457   4500000000100000008004100001260001500042100001800057700003000075700002200105700001800127700002500145700002200170700001600192700001700208700001800225700002200243700001500265700002100280700002300301700001900324700002000343700002600363700001700389700001600406700002400422700002100446700002100467700002800488700001800516700001800534700002000552700001900572700001600591700002200607245010400629856004300733300001200776520104000788022001401828       2023                            d  c2023-09-051 aRobert Jordan1 aStephanie L Ford-Scheimer1 aRodolfo M Alarcon1 aAnthony Atala1 aJeffrey T Borenstein1 aKyle R Brimacombe1 aSara Cherry1 aHans Clevers1 aMindy I Davis1 aSimon G P Funnell1 aLee Gehrke1 aLinda G Griffith1 aAbigail C Grossman1 aThomas Hartung1 aDonald E Ingber1 aNicole C Kleinstreuer1 aCalvin J Kuo1 aEmily M Lee1 aChristine L Mummery1 aThames E Pickett1 aSasirekha Ramani1 aEdwin A Rosado-Olivieri1 aEvi B Struble1 aZhengpeng Wan1 aMark S Williams1 aMatthew D Hall1 aMarc Ferrer1 aSarine Markossian00aReport of the Assay Guidance Workshop on 3-Dimensional Tissue Models for Antiviral Drug Development  uhttps://doi.org/10.1093/infdis/jiad334  ajiad3343 aThe National Center for Advancing Translational Sciences (NCATS) Assay Guidance Manual (AGM) Workshop on 3D Tissue Models for Antiviral Drug Development, held virtually on 7–8 June 2022, provided comprehensive coverage of critical concepts intended to help scientists establish robust, reproducible, and scalable 3D tissue models to study viruses with pandemic potential. This workshop was organized by NCATS, the National Institute of Allergy and Infectious Diseases, and the Bill and Melinda Gates Foundation. During the workshop, scientific experts from academia, industry, and government provided an overview of 3D tissue models’ utility and limitations, use of existing 3D tissue models for antiviral drug development, practical advice, best practices, and case studies about the application of available 3D tissue models to infectious disease modeling. This report includes a summary of each workshop session as well as a discussion of perspectives and challenges related to the use of 3D tissues in antiviral drug discovery.  a0022-1899