02357nas a2200253   4500000000100000008004100001260001500042653001500057653003100072653002000103653002900123653001400152100002200166700001400188700001600202700001700218700001700235245008600252856007200338300001200410490000700422520166000429022001402089       2023                            d  c2023-03-0110a-mimicking10a3D engineered tissue model10aInfection route10aInfectious viral disease10aPathology1 aKyeong Seob Hwang1 aEun U Seo1 aNakwon Choi1 aJongbaeg Kim1 aHong Nam Kim00a3D engineered tissue models for studying human-specific infectious viral diseases  uhttps://www.sciencedirect.com/science/article/pii/S2452199X22003942  a576-5940 v213 aViral infections cause damage to various organ systems by inducing organ-specific symptoms or systemic multi-organ damage. Depending on the infection route and virus type, infectious diseases are classified as respiratory, nervous, immune, digestive, or skin infections. Since these infectious diseases can widely spread in the community and their catastrophic effects are severe, identification of their causative agent and mechanisms underlying their pathogenesis is an urgent necessity. Although infection-associated mechanisms have been studied in two-dimensional (2D) cell culture models and animal models, they have shown limitations in organ-specific or human-associated pathogenesis, and the development of a human-organ-mimetic system is required. Recently, three-dimensional (3D) engineered tissue models, which can present human organ-like physiology in terms of the 3D structure, utilization of human-originated cells, recapitulation of physiological stimuli, and tight cell–cell interactions, were developed. Furthermore, recent studies have shown that these models can recapitulate infection-associated pathologies. In this review, we summarized the recent advances in 3D engineered tissue models that mimic organ-specific viral infections. First, we briefly described the limitations of the current 2D and animal models in recapitulating human-specific viral infection pathology. Next, we provided an overview of recently reported viral infection models, focusing particularly on organ-specific infection pathologies. Finally, a future perspective that must be pursued to reconstitute more human-specific infectious diseases is presented.  a2452-199X