01686nas a2200289   4500000000100000000000100001008004100002260001500043100001700058700001700075700001800092700001600110700001900126700002600145700001900171700002500190700002300215700002200238700001900260700001600279245007800295856006000373300001000433490000700443520093200450022001401382       2022                            d  c2022-01-121 aDavid Pamies1 aMarcel Leist1 aSandra Coecke1 aGerard Bowe1 aDavid G. Allen1 aGerhard Gstraunthaler1 aAnna Bal-Price1 aFrancesca Pistollato1 aRob B. M. De Vries1 aHelena T. Hogberg1 aThomas Hartung1 aGlyn Stacey00aGuidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0)  uhttps://www.altex.org/index.php/altex/article/view/2376  a30-700 v393 aGood Cell and Tissue Culture Practice (GCCP) 2.0 is an updated guidance document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical use in the laboratory to assure the reproducibility of in vitro (cell-based) work. The update in the guidance was essential as cell models have advanced dramatically to more complex culture systems and need more comprehensive quality management to ensure reproducibility and high-quality scientific data. This document describes six main principles to consider when performing cell culture including characterization and maintenance of essential characteristics, quality management, documentation and reporting, safety, education and training, and ethics. The document does not intend to impose detailed procedures but to describe potential quality issues. It is foreseen that the document will require further updates as the science and technologies evolve over time.  a1868-8551