01262nas a2200241   4500000000100000000000100001008004100002260001500043653001200058653001100070653002300081653002200104653001400126653002700140653002500167653001500192100001700207245005200224300001400276490000800290520070800298022001401006       2016                            d  c2016-06-1610aAnimals10aHumans10aModels, Biological10aOrgan Specificity10aorganoids10aSequence Analysis, RNA10aSingle-Cell Analysis10aStem cells1 aHans Clevers00aModeling Development and Disease with Organoids  a1586-15970 v1653 aRecent advances in 3D culture technology allow embryonic and adult mammalian stem cells to exhibit their remarkable self-organizing properties, and the resulting organoids reflect key structural and functional properties of organs such as kidney, lung, gut, brain and retina. Organoid technology can therefore be used to model human organ development and various human pathologies 'in a dish." Additionally, patient-derived organoids hold promise to predict drug response in a personalized fashion. Organoids open up new avenues for regenerative medicine and, in combination with editing technology, for gene therapy. The many potential applications of this technology are only beginning to be explored.  a1097-4172