01767nas a2200229   4500000000100000000000100001008004100002260001500043653001500058653002200073653001800095653003000113653002000143100003000163700001800193245008000211856007200291300001100363490000600374520114300380022001401523       2022                            d  c2022-12-0110a3D culture10aIn vitro modeling10amicrofluidics10amicrophysiological system10aorgan-on-a-chip1 aMaría Virumbrales-Muñoz1 aJose M. Ayuso00aFrom microfluidics to microphysiological systems: Past, present, and future  uhttps://www.sciencedirect.com/science/article/pii/S2666102022000015  a1000150 v43 aFor over a decade, we have seen significant strides in the microfluidics field that have led to the concept of microphysiological systems. These systems emerged in the early 2010s as versatile in vitro platforms that allowed researchers to mimic tissue complexity in vitro. Early models focused on showing the advantages of fluid physics at the microscale and demonstrating proof-of-concept experiments. As the technology evolved, microfluidic models became more complex and showed their capacity to mimic complex biological responses at an organ level, coining the concept of organ-on-a-chip platforms. Gathered under the banner of “microphysiological systems”, current platforms evaluate complex dynamics that involve numerous cell types in highly organized scenarios. Recent models have leveraged advanced imaging and multi-omics techniques to study a large variety of cellular and molecular processes, from cancer and strokes to reproductive biology and infectious diseases. In this piece, we highlight the main hallmarks of each of these periods and outline current and upcoming trends in the field of microphysiological systems.  a2666-1020