@article{631,
  author = {Alexandra Sontheimer-Phelps and David B. Chou and Alessio Tovaglieri and Thomas C. Ferrante and Taylor Duckworth and Cicely Fadel and Viktoras Frismantas and Arlene D. Sutherland and Sasan Jalili-Firoozinezhad and Magdalena Kasendra and Eric Stas and James C. Weaver and Camilla A. Richmond and Oren Levy and Rachelle Prantil-Baun and David T. Breault and Donald E. Ingber},
  title = {Human Colon-on-a-Chip Enables Continuous In Vitro Analysis of Colon Mucus Layer Accumulation and Physiology},
  abstract = {METHODS: A human colon-on-a-chip (Colon Chip) microfluidic device lined by primary patient-derived colonic epithelial cells was used to recapitulate mucus bilayer formation, and to visualize mucus accumulation in living cultures noninvasively.
RESULTS: The Colon Chip supports spontaneous goblet cell differentiation and accumulation of a mucus bilayer with impenetrable and penetrable layers, and a thickness similar to that observed in the human colon, while maintaining a subpopulation of proliferative epithelial cells. Live imaging of the mucus layer formation on-chip showed that stimulation of the colonic epithelium with prostaglandin E2, which is increased during inflammation, causes rapid mucus volume expansion via an Na-K-Cl cotransporter 1 ion channel–dependent increase in its hydration state, but no increase in de novo mucus secretion.
CONCLUSIONS: This study shows the production of colonic mucus with a physiologically relevant bilayer structure in vitro, which can be analyzed in real time noninvasively. The Colon Chip may offer a new preclinical tool to analyze},
  year = {2020},
  journal = {Cellular and Molecular Gastroenterology and Hepatology},
  volume = {9},
  pages = {507-526},
  month = {2020},
  issn = {2352345X},
  url = {https://linkinghub.elsevier.com/retrieve/pii/S2352345X19301638},
  doi = {10.1016/j.jcmgh.2019.11.008},
  language = {en},
}