@article{4171,
  keywords = {Biomedical Engineering, Developmental biology, Heart development, RNA sequencing, Stem cells},
  author = {Brett Volmert and Artem Kiselev and Aniwat Juhong and Fei Wang and Ashlin Riggs and Aleksandra Kostina and Colin O’Hern and Priyadharshni Muniyandi and Aaron Wasserman and Amanda Huang and Yonatan Lewis-Israeli and Vishal Panda and Sudin Bhattacharya and Adam Lauver and Sangbum Park and Zhen Qiu and Chao Zhou and Aitor Aguirre},
  title = {A patterned human primitive heart organoid model generated by pluripotent stem cell self-organization},
  abstract = {Pluripotent stem cell-derived organoids can recapitulate significant features of organ development in vitro. We hypothesized that creating human heart organoids by mimicking aspects of in utero gestation (e.g., addition of metabolic and hormonal factors) would lead to higher physiological and anatomical relevance. We find that heart organoids produced using this self-organization-driven developmental induction strategy are remarkably similar transcriptionally and morphologically to age-matched human embryonic hearts. We also show that they recapitulate several aspects of cardiac development, including large atrial and ventricular chambers, proepicardial organ formation, and retinoic acid-mediated anterior-posterior patterning, mimicking the developmental processes found in the post-heart tube stage primitive heart. Moreover, we provide proof-of-concept demonstration of the value of this system for disease modeling by exploring the effects of ondansetron, a drug administered to pregnant women and associated with congenital heart defects. These findings constitute a significant technical advance for synthetic heart development and provide a powerful tool for cardiac disease modeling.},
  year = {2023},
  journal = {Nature Communications},
  volume = {14},
  pages = {8245},
  month = {2023-12-12},
  issn = {2041-1723},
  url = {https://www.nature.com/articles/s41467-023-43999-1},
  doi = {10.1038/s41467-023-43999-1},
  language = {en},
}