@article{3531,
  keywords = {Adult, aging, Animals, Child, Crohn Disease, Datasets as Topic, Enteric Nervous System, Epithelial Cells, Female, Fetus, Health, Humans, Intestines, Lymph Nodes, Mice, Mice, Inbred C57BL, Organogenesis, Receptors, IgG, Signal Transduction, Spatio-Temporal Analysis, Time Factors},
  author = {Rasa Elmentaite and Natsuhiko Kumasaka and Kenny Roberts and Aaron Fleming and Emma Dann and Hamish W. King and Vitalii Kleshchevnikov and Monika Dabrowska and Sophie Pritchard and Liam Bolt and Sara F. Vieira and Lira Mamanova and Ni Huang and Francesca Perrone and Issac Goh Kai'En and Steven N. Lisgo and Matilda Katan and Steven Leonard and Thomas R. W. Oliver and C. Elizabeth Hook and Komal Nayak and Lia S. Campos and Cecilia Domínguez Conde and Emily Stephenson and Justin Engelbert and Rachel A. Botting and Krzysztof Polanski and Stijn van Dongen and Minal Patel and Michael D. Morgan and John C. Marioni and Omer Ali Bayraktar and Kerstin B. Meyer and Xiaoling He and Roger A. Barker and Holm H. Uhlig and Krishnaa T. Mahbubani and Kourosh Saeb-Parsy and Matthias Zilbauer and Menna R. Clatworthy and Muzlifah Haniffa and Kylie R. James and Sarah A. Teichmann},
  title = {Cells of the human intestinal tract mapped across space and time},
  abstract = {The cellular landscape of the human intestinal tract is dynamic throughout life, developing in utero and changing in response to functional requirements and environmental exposures. Here, to comprehensively map cell lineages, we use single-cell RNA sequencing and antigen receptor analysis of almost half a million cells from up to 5 anatomical regions in the developing and up to 11 distinct anatomical regions in the healthy paediatric and adult human gut. This reveals the existence of transcriptionally distinct BEST4 epithelial cells throughout the human intestinal tract. Furthermore, we implicate IgG sensing as a function of intestinal tuft cells. We describe neural cell populations in the developing enteric nervous system, and predict cell-type-specific expression of genes associated with Hirschsprung's disease. Finally, using a systems approach, we identify key cell players that drive the formation of secondary lymphoid tissue in early human development. We show that these programs are adopted in inflammatory bowel disease to recruit and retain immune cells at the site of inflammation. This catalogue of intestinal cells will provide new insights into cellular programs in development, homeostasis and disease.},
  year = {2021},
  journal = {Nature},
  volume = {597},
  pages = {250-255},
  month = {2021-09},
  issn = {1476-4687},
  doi = {10.1038/s41586-021-03852-1},
  language = {eng},
}