@article{3376,
  keywords = {Assembloids, cerebral cavernous malformations, human PSC-derived organoids, human blood-brain barrier, neuro-vascular development, neuro-vascular interactions, single-cell transcriptomics, spatial transcriptomics},
  author = {Lan Dao and Zhen You and Lu Lu and Tianyang Xu and Avijite Kumer Sarkar and Hui Zhu and Miao Liu and Riccardo Calandrelli and George Yoshida and Pei Lin and Yifei Miao and Sarah Mierke and Srijan Kalva and Haining Zhu and Mingxia Gu and Sudhakar Vadivelu and Sheng Zhong and L. Frank Huang and Ziyuan Guo},
  title = {Modeling blood-brain barrier formation and cerebral cavernous malformations in human PSC-derived organoids},
  abstract = {The human blood-brain barrier (hBBB) is a highly specialized structure that regulates passage across blood and central nervous system (CNS) compartments. Despite its critical physiological role, there are no reliable in vitro models that can mimic hBBB development and function. Here, we constructed hBBB assembloids from brain and blood vessel organoids derived from human pluripotent stem cells. We validated the acquisition of blood-brain barrier (BBB)-specific molecular, cellular, transcriptomic, and functional characteristics and uncovered an extensive neuro-vascular crosstalk with a spatial pattern within hBBB assembloids. When we used patient-derived hBBB assembloids to model cerebral cavernous malformations (CCMs), we found that these assembloids recapitulated the cavernoma anatomy and BBB breakdown observed in patients. Upon comparison of phenotypes and transcriptome between patient-derived hBBB assembloids and primary human cavernoma tissues, we uncovered CCM-related molecular and cellular alterations. Taken together, we report hBBB assembloids that mimic the core properties of the hBBB and identify a potentially underlying cause of CCMs.},
  year = {2024},
  journal = {Cell Stem Cell},
  month = {2024-05-15},
  issn = {1934-5909},
  url = {https://www.sciencedirect.com/science/article/pii/S1934590924001462},
  doi = {10.1016/j.stem.2024.04.019},
}