@article{2921,
  keywords = {Experimental models of disease, Regenerative Medicine, Stem cells, Stem-cell research},
  author = {Mattia Francesco Maria Gerli and Giuseppe Calà and Max Arran Beesley and Beatrice Sina and Lucinda Tullie and Kylin Yunyan Sun and Francesco Panariello and Federica Michielin and Joseph R. Davidson and Francesca Maria Russo and Brendan C. Jones and Dani Do Hyang Lee and Savvas Savvidis and Theodoros Xenakis and Ian C. Simcock and Anna A. Straatman-Iwanowska and Robert A. Hirst and Anna L. David and Christopher O’Callaghan and Alessandro Olivo and Simon Eaton and Stavros P. Loukogeorgakis and Davide Cacchiarelli and Jan Deprest and Vivian S. W. Li and Giovanni Giuseppe Giobbe and Paolo De Coppi},
  title = {Single-cell guided prenatal derivation of primary fetal epithelial organoids from human amniotic and tracheal fluids},
  abstract = {Isolation of tissue-specific fetal stem cells and derivation of primary organoids is limited to samples obtained from termination of pregnancies, hampering prenatal investigation of fetal development and congenital diseases. Therefore, new patient-specific in vitro models are needed. To this aim, isolation and expansion of fetal stem cells during pregnancy, without the need for tissue samples or reprogramming, would be advantageous. Amniotic fluid (AF) is a source of cells from multiple developing organs. Using single-cell analysis, we characterized the cellular identities present in human AF. We identified and isolated viable epithelial stem/progenitor cells of fetal gastrointestinal, renal and pulmonary origin. Upon culture, these cells formed clonal epithelial organoids, manifesting small intestine, kidney tubule and lung identity. AF organoids exhibit transcriptomic, protein expression and functional features of their tissue of origin. With relevance for prenatal disease modeling, we derived lung organoids from AF and tracheal fluid cells of congenital diaphragmatic hernia fetuses, recapitulating some features of the disease. AF organoids are derived in a timeline compatible with prenatal intervention, potentially allowing investigation of therapeutic tools and regenerative medicine strategies personalized to the fetus at clinically relevant developmental stages.},
  year = {2024},
  journal = {Nature Medicine},
  pages = {1-13},
  month = {2024-03-04},
  issn = {1546-170X},
  url = {https://www.nature.com/articles/s41591-024-02807-z},
  doi = {10.1038/s41591-024-02807-z},
  language = {en},
}