@article{2046,
  keywords = {dorsoventral organization, human pluripotent stem cells, microcontact printing, spinal cord organoid, symmetry breaking},
  author = {Kyubin Seo and Subin Cho and Hyogeun Shin and Aeri Shin and Ju-Hyun Lee and June Hoan Kim and Boram Lee and Hwanseok Jang and Youngju Kim and Hyo Min Cho and Yongdoo Park and Hee Youn Kim and Taeseob Lee and Woong-Yang Park and Yong Jun Kim and Esther Yang and Dongho Geum and Hyun Kim and Il-Joo Cho and Sanghyuk Lee and Jae Ryun Ryu and Woong Sun},
  title = {Symmetry Breaking of Human Pluripotent Stem Cells (hPSCs) in Micropattern Generates a Polarized Spinal Cord-Like Organoid (pSCO) with Dorsoventral Organization},
  abstract = {Axis formation and related spatial patterning are initiated by symmetry breaking during development. A geometrically confined culture of human pluripotent stem cells (hPSCs) mimics symmetry breaking and cell patterning. Using this, polarized spinal cord organoids (pSCOs) with a self-organized dorsoventral (DV) organization are generated. The application of caudalization signals promoted regionalized cell differentiation along the radial axis and protrusion morphogenesis in confined hPSC colonies. These detached colonies grew into extended spinal cord-like organoids, which established self-ordered DV patterning along the long axis through the spontaneous expression of polarized DV patterning morphogens. The proportions of dorsal/ventral domains in the pSCOs can be controlled by the changes in the initial size of micropatterns, which altered the ratio of center-edge cells in 2D. In mature pSCOs, highly synchronized neural activity is separately detected in the dorsal and ventral side, indicating functional as well as structural patterning established in the organoids. This study provides a simple and precisely controllable method to generate spatially ordered organoids for the understanding of the biological principles of cell patterning and axis formation during neural development.},
  year = {2023},
  journal = {Advanced Science},
  volume = {10},
  pages = {2301787},
  month = {2023},
  issn = {2198-3844},
  url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202301787},
  doi = {10.1002/advs.202301787},
  language = {en},
}