@article{2041,
  keywords = {afterload, cardiac in vitro models, force application on cells, magnetic gels, preload},
  author = {Moran Yadid and Mario Hagel and Megan Beldjilali Labro and Baptiste Le Roi and Carina Flaxer and Eli Flaxer and A. Ronny Barnea and Shai Tejman-Yarden and Eric Silberman and Xin Li and Rossana Rauti and Yael Leichtmann-Bardoogo and Hongyan Yuan and Ben M. Maoz},
  title = {A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films},
  abstract = {Despite significant advancements in in vitro cardiac modeling approaches, researchers still lack the capacity to obtain in vitro measurements of a key indicator of cardiac function: contractility, or stroke volume under specific loading conditions—defined as the pressures to which the heart is subjected prior to and during contraction. This work puts forward a platform that creates this capability, by providing a means of dynamically controlling loading conditions in vitro. This dynamic tissue loading platform consists of a thin magnetoresponsive hydrogel cantilever on which 2D engineered myocardial tissue is cultured. Exposing the cantilever to an external magnetic field—generated by positioning magnets at a controlled distance from the cantilever—causes the hydrogel film to stretch, creating tissue load. Next, cell contraction is induced through electrical stimulation, and the force of the contraction is recorded, by measuring the cantilever's deflection. Force–length-based measurements of contractility are then derived, comparable to clinical measurements. In an illustrative application, the platform is used to measure contractility both in untreated myocardial tissue and in tissue exposed to an inotropic agent. Clear differences are observed between conditions, suggesting that the proposed platform has significant potential to provide clinically relevant measurements of contractility.},
  year = {23},
  journal = {Advanced Science},
  pages = {2207498},
  month = {23 July 2023},
  issn = {2198-3844},
  url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202207498},
  doi = {10.1002/advs.202207498},
  language = {en},
}