@article{1466,
  keywords = {Adult, BRCA1 Protein, Breast Neoplasms, Cell Culture Techniques, Cell Differentiation, Cell Lineage, Epidermal Growth Factor, ErbB Receptors, Female, Humans, Mammary Glands, Human, Middle Aged, organoids, Single-Cell Analysis, Stem cells, Transforming Growth Factor beta, Young Adult, p38 Mitogen-Activated Protein Kinases},
  author = {Jennifer M. Rosenbluth and Ron C. J. Schackmann and G. Kenneth Gray and Laura M. Selfors and Carman Man-Chung Li and Mackenzie Boedicker and Hendrik J. Kuiken and Andrea Richardson and Jane Brock and Judy Garber and Deborah Dillon and Norman Sachs and Hans Clevers and Joan S. Brugge},
  title = {Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages},
  abstract = {Recently, organoid technology has been used to generate a large repository of breast cancer organoids. Here we present an extensive evaluation of the ability of organoid culture technology to preserve complex stem/progenitor and differentiated cell types via long-term propagation of normal human mammary tissues. Basal/stem and luminal progenitor cells can differentiate in culture to generate mature basal and luminal cell types, including ER+ cells that have been challenging to maintain in culture. Cells associated with increased cancer risk can also be propagated. Single-cell analyses of matched organoid cultures and native tissues by mass cytometry for 38 markers provide a higher resolution representation of the multiple mammary epithelial cell types in the organoids, and demonstrate that protein expression patterns of the tissue of origin can be preserved in culture. These studies indicate that organoid cultures provide a valuable platform for studies of mammary differentiation, transformation, and breast cancer risk.},
  year = {2020},
  journal = {Nature Communications},
  volume = {11},
  pages = {1711},
  month = {2020-04-06},
  issn = {2041-1723},
  doi = {10.1038/s41467-020-15548-7},
  language = {eng},
}